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. 2020 Aug 14;11:4075. doi: 10.1038/s41467-020-17942-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Bar graph showing the number of genes differentially expressed (>twofold change, adjusted P value <0.05) between young and old stromal cells of the indicated populations. b Heatmap of Metacore Process Networks enriched in three or more aged stromal cell populations at P < 0.005. c Quantification of myelo-erythroid progenitors from old mice treated with control IgG (IgG; N = 9) and neutralising anti-IL-6 antibody (αIL-6; N = 10). Data are from five independent experiments. Values are mean ± s.d. *P < 0.05; **P < 0.01; ***P < 0.001 (two-tailed unpaired Student’s t test). Exact P values: MkP: 0.24; preGM: 0.24; GMP: 0.03; preMegE: 0.02; preCFU-E: 0.82; CFU-E: 0.85. d In vitro colony-forming potential of LK (defined as Lin⁻Sca-1⁻c-Kit⁺ cells) isolated from BM of old mice after injection of IgG isotype control (N = 4) or αIL-6 Ab (N = 4) assayed under E condition (M3436; 500 cells). Data are from two different experiments. Values show mean ± s.e.m. ***P < 0.001 (two-tailed unpaired Student’s t test). Exact P value: 0.000000005. e In vitro colony-forming potential of LK (defined as Lin⁻Sca-1⁻c-Kit⁺ cells) isolated from BM of old mice after injection of IgG isotype control (N = 4) or αIL-6 Ab (N = 4) assayed under GM condition (M3534; 500 cells). Data are from two different experiments. Values show mean ± s.e.m. ***P < 0.001 (two-tailed unpaired Student’s t test). Exact P value: 0.0005. f In vitro colony-forming potential of preCFU-E cells isolated from BM of old mice after injection of IgG isotype control (N = 4) or αIL-6 Ab (N = 4) assayed under E condition (M3436; 500 cells). Data are from two different experiments. Values show mean ± s.e.m. ***P < 0.001 (two-tailed unpaired Student’s t test). Exact P value = 0.0005. g GSEA analysis comparing expression of preCFU-E-specific genes in preCFU-Es isolated from young and old IgG-treated mice. The normalised enrichment score (NES) and P value are shown. h GSEA analysis comparing expression of preCFU-E-specific genes in preCFU-Es isolated from young and old anti-IL-6-treated CFU-Es. The normalised enrichment score (NES) and P value are shown. Source data are provided as a source data file.