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. 2020 Aug 14;11:4075. doi: 10.1038/s41467-020-17942-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a GSEA analysis of MPP2-specific genes in LSK cells from vehicle (VEH) and SB-treated old mice. Normalised enrichment score (NES) and P value are shown (P < 0.001). b GSEA analysis of MPP4-specific genes in LSK cells isolated from vehicle (VEH) and SB-treated old mice. Normalised enrichment score (NES) and P value are shown (P < 0.001). c tSNE plot of vehicle and SB-treated single LSK cell transcriptomes. d GSEA analysis of MPP4-specific genes in computationally defined MPP4 cells isolated from vehicle and SB-treated old mice. The normalised enrichment score (NES) and P value are shown (P = 0.014). e Fraction of LSK cultures from vehicle and SB-treated old mice (10 cells plated/culture) positive for B-lymphocytes after 3 weeks of culture. N = 192 cultures/condition, two independent experiments. ***P < 0.001 (two-sided χ² test). Exact P value: 0.0004. f In vitro colony-forming potential of B precursor cells isolated young mice (N = 6), vehicle (N = 18) and SB-treated (N = 15) old mice after plating 25.000 cells in M3630 medium. Data from two different experiments. Values show mean ± s.e.m. **P < 0.01; ***P < 0.001 (two-tailed unpaired Student’s t test). Exact P values: VEH-SB: 0.0000000002; SB-Young: 0.0017; VEH-Young: 0.0000000002. g PB lineage contribution of LT-HSCs from vehicle (VEH) and SB-treated (SB) old mice in transplanted recipients (VEH: N = 19; SB: N = 20; three independent experiments). Mean values ± s.e.m are shown. **P < 0.01; ***P < 0.001 (two-sided Mann–Whitney U-test). Exact P values: PLT: 0.000002; ERY: 0.002; MYL: 0.002; BLY: 0.66; TLY: 0.41. h Donor HSC PB contribution of LT-HSCs isolated from vehicle (VEH) and SB-treated (SB) old mice in transplanted recipients (VEH: N = 19; SB: N = 20; three independent experiments) from g normalised to platelet output. Mean values ± s.e.m are shown. *P < 0.05; **P < 0.01 (two-sided Mann–Whitney U-test). Exact P values: PLT: not applicable; ERY: 0.01; MYL: 0.38; BLY: 0.006; TLY: 0.002. P values for the comparison of each lineage output to platelet output for the same condition are also shown: ^#P < 0.05; ^###P < 0.001 (two-sided Mann–Whitney U-test). Exact P values, VEH condition: ERY: 0.36; MYL: 0.02; BLY: 0.0001; TLY: 0.000002. Exact P values, SB condition: ERY: 0.31; MYL: 0.55; BLY: 0.74; TLY: 0.03. Source data are provided as a source data file.