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. 2020 Aug 14;11:4106. doi: 10.1038/s41467-020-17853-7

Fig. 2. Structural intermediates of ribosome rescue by ArfB as visualized by cryo-EM.

Fig. 2

For cryo-EM analysis, ArfB was trapped on the ribosome using the antimicrobial peptide Api137. a Major states of ArfB-bound P + 0 and P + 9 complexes exhibit a striking structural similarity. Close-ups: Superposition of P + 0 (lighter colors) and P + 9 (darker colors) structures in the PTC (top) and the DC region (bottom). b Stalled P + 9 complex with dipeptidyl-tRNA prior to ArfB binding. c Post-hydrolysis P + 9 complex with P/E hybrid tRNA. Note the lack of density for mRNA in the mRNA entry channel in the ArfB-bound P + 9 states indicating a highly flexible 3′ extension of mRNA versus the well-defined 3′ extension in the vacant P + 9 complex.