Table 2.
Liver and intestine antioxidant enzymes activity of gilthead sea bream fed the experimental diets.
| Organ | Liver | Intestine | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Diets | A | B | C | D | SEM | A | B | C | D | SEM |
| 2.0/0.2/0.1 | 1.0/0.4/0.4 | 0/0.6/0.6 | 0/0.3/1.5 | 2.0/0.2/0.1 | 1.0/0.4/0.4 | 0/0.6/0.6 | 0/0.3/1.5 | |||
| CAT | 56.7 | 51.8 | 42.1 | 48.5 | 1.68 | 153.8 | 92.4 | 76.6 | 158.6 | 16.0 |
| G6PDH | 136.6 | 132.1 | 127.8 | 110.0 | 5.25 | 24.6 | 25.8 | 31.0 | 20.2 | 1.66 |
| GR | 8.8 | 7.3 | 7.0 | 6.6 | 0.28 | 20.7 | 15.9 | 14.9 | 16.1 | 0.96 |
| SOD | 106.9 | 126.9 | 110.3 | 108.5 | 6.59 | 1,341.4 | 1,220.3 | 1,232.5 | 929.0 | 63.7 |
| GPX | 54.2 | 36.4 | 38.5 | 35.5 | 3.22 | 10.2 | 9.6 | 9.6 | 9.0 | 0.64 |
| Variance source | Diets | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Diet | Organ | Interaction | A | B | C | D | ||||
| Two-way Anova | ||||||||||
| CAT | 0.022 | 0.000 | ns | – | – | – | – | |||
| G6PDH | Ns | 0.000 | ns | – | – | – | – | |||
| GR | 0.008 | 0.000 | ns | b | ab | a | a | |||
| SOD | Ns | 0.000 | ns | – | – | – | – | |||
| GPX | Ns | 0.000 | ns | – | – | – | – | |||
Values presented as means (n = 9 for liver and n = 6 for intestine) and pooled standard error of the mean (SEM). Enzyme activities expressed as mU mg protein−1 for G6PDH, GR, and GPX and as U mg protein−1 for CAT and SOD
Two-way ANOVA: ns: non-significant (P ≥ 0.05).