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. Author manuscript; available in PMC: 2020 Aug 14.
Published in final edited form as: Cell Rep. 2020 Jul 21;32(3):107939. doi: 10.1016/j.celrep.2020.107939

Figure 3. New Groups from the Human Oral Cavity Are Ultra-small Epibionts.

Figure 3.

(A) Human saliva and tongue samples were stained using fluorescence in situ hybridization (FISH) probes that specifically targeted the G1, G3, G5, and G6 lineages of TM7 phylum, as well as the SR1 phylum. Column 1 is TM7 staining with lineage-specific probes (red), column 2 is total DNA stain with Syto9 dye (green), and column 3 is merged images (magnified images indicate ultra-small bacteria). All scale bars are 5 μm.

(B) PCR amplification of G3, G5, G6, and SR1 16S rRNA with lineage-specific primers, using template DNA isolated from original saliva samples, saliva supernatants, and filtrate passing through 0.45 μm filters (see STAR Methods) confirming ultra-small cell sizes.