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. 2020 Aug 14;11:4107. doi: 10.1038/s41467-020-17881-3

Fig. 2. The loss of DGAT1 function decreases TG accumulation, LDs, and inflammatory function.

Fig. 2

a Effect of DGAT1 inhibitor T863 (DGAT1i) on total TG, and b distinct TG species (n = 4 biologically independent samples) in resting (M0) and macrophages stimulated with IFNγ + LPS. c Bodipy 493/503 fluorescence visualized by fluorescence microscopy (scale bars represent 10 μm) or d measured by flow cytometry (MFI, median fluorescence intensity) (n = 3 biologically independent samples). e C16 Bodipy uptake (n = 4 biologically independent samples). f Effect of DGAT1i on DG levels in M0 or macrophages stimulated with IFNγ + LPS (n = 4 biologically independent samples). g Effect of Dgat1-shRNA (shDgat1) or empty vector (EV) on TG species. h Bodipy 493/503 fluorescence in macrophages transduced with shDgat1 or EV and treated with IFNγ + LPS (n = 3 biologically independent samples). i Pro IL-1β positive macrophages stimulated with IFNγ + LPS, plus/minus DGATi or j shDgat1 (n = 4 biologically independent samples). k, l Effect of the loss of DGAT1 function on IL-6 production (k n = 8 and l n = 5 biologically independent samples). m Effect of DGAT1i on phagocytosis of S. aureus (PE-labelled) (n = 4 biologically independent samples). n C57BL/6 mice were injected with DGAT1i, or carrier, 30 min before receiving 8 mg kg−1 of i.p. LPS or PBS. Drop in body temperature was calculated in relation to the initial body temperature. o Bodipy 493/503 fluorescence in large (F4/80+TIM4+), and p small (F4/80+TIM4neg), peritoneal macrophages from the mice mice shown in (n), recovered at 10 h post injection of LPS or PBS (n = 5 biologically independent samples). q Serum IL-1β at 2 h post injection (n = 4 biologically independent samples). r Serum IL-6 at 10 h post injection (n = 5 biologically independent samples). For a–g and k–m, assays were at 18 h post activation; i, j at 6 h. Data are represented as mean values ± s.e.m. One-way ANOVA with Bonferroni’s multiple comparison test (a, e, f, n); Unpaired two-tailed Student’s t test (d, h–m, p–r). (a, b, e–h, n–r) representative of two or (c, d, i–m) three experiments. Source data are provided as a Source Data file.