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. 2020 Aug 14;10:13844. doi: 10.1038/s41598-020-70757-w

Figure 2.

Figure 2

Real-time analysis of circadian expression of PER2::LUC in ex vivo DRG cultures. (A) Representative records of bioluminescence (LumiCycle Analysis, v. 3.0002, Actimetrics) showing circadian profiles of PER2::LUC expression from DRG1 to DRG5 isolated from Per2::LucSV mice (n = 48, 8–9 each for DRG L1–L5). DRGs were explanted before light off. (B) Circadian oscillation of DRG explants persist rhythmicity after media change. After day 7 of culture, media were replaced and circadian oscillation was reinitiated in L4 DRG explants (n = 3).