Table 1.
Melting temperature (Tm) and basal activity of PLCε variants.
| PLCε variant | Tm ± SD (˚C) | Significance | n | Specific activity ± S.D. (nmol IP3/min/ nmol PLCε variant) | Significance | n |
|---|---|---|---|---|---|---|
| PH-COOHa | 51.9 ± 0.9 | 5 | 360 ± 120 | 9 | ||
| PH-COOH Δ RA1 | 47.6 ± 1.0 | **** | 3 | 16 ± 4 | **** | 5 |
| PH-RA1 | 50.8 ± 0.3 | 4 | 200 ± 50 | * | 9 | |
| PH-C2a | 48.3 ± 1.0 | **** | 4 | 78 ± 20 | *** | 5 |
| EF3-RA1 | 52.7 ± 0.4 | 4 | 230 ± 30 | 3 | ||
| 1526–1546 | 51.0 ± 0.2 | 3 | 590 ± 40 | ** | 4 | |
| 1621–1634 | 50.8 ± 0.3 | 3 | 290 ± 130 | 3 | ||
| N1316E | 48.8 ± 0.4 | **** | 4 | 240 ± 120 | 3 | |
| D1911A | 49.7 ± 0.4 | **** | 4 | 630 ± 120 | *** | 4 |
| R1965A | 50.3 ± 0.6 | ** | 3 | 640 ± 60 | * | 2 |
| F1982A | 50.2 ± 0.3 | *** | 4 | 390 ± 40 | 3 | |
| F1982E | 50.2 ± 0.4 | ** | 3 | 990 ± 300 | **** | 3 |
| F2006A | 48.9 ± 0.3 | **** | 3 | 330 ± 100 | 3 | |
| F2006E | 46.6 ± 0.7 | **** | 4 | 320 ± 50 | 3 | |
| F2077A | 50.4 ± 0.4 | ** | 4 | 400 ± 120 | 3 | |
| R2085A | 49.4 ± 0.2 | **** | 3 | 410 ± 70 | 3 |
aData previously reported22.
Results are based on one-way ANOVA followed by Dunnett’s multiple comparisons test vs. PLCε PH-COOH. (****p ≤ 0.0001,***p ≤ 0.0005, **p ≤ 0.001, *p ≤ 0.05).