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. 2020 Aug 14;11:4100. doi: 10.1038/s41467-020-17984-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Schematic illustration of the nanofiber formation (sol–gel transition) through oxime formation. b Macroscopic sol–gel transition of Ald-F(F)F upon (top) O-benzylhydroxylamine and (bottom) buffer treatment. c Rheological properties of the hydrogel. G’: storage shear modulus, G’’: loss shear modulus. Frequency: 10 rad/s, strain amplitude: 1%. d HPLC analysis of the formation rate of BnOx-F(F)F. Internal standard: fluorescein. e High-resolution Airyscan CLSM images (left) before and 1 h after addition of (middle) O-benzylhydroxylamine or (right) buffer to Ald-F(F)F. Condition: [Ald-F(F)F] = 4.3 mM (0.20 wt%), [O-benzylhydroxylamine] = 4.3 mM (1.0 eq), [NP-Alexa647] = 4.0 µM, 100 mM MES, pH 6.0.