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. 2020 Aug 14;11:4100. doi: 10.1038/s41467-020-17984-x

Fig. 5. Formation of the parallel self-sorting network through the oxime exchange protocol.

Fig. 5

a Schematic illustration of the transformation from the interpenetrated SDN to the parallel SDN by the oxime-exchange protocol. b High-resolution Airyscan CLSM images of (top) the interpenetrated SDN, (middle) the single lipid network, and (bottom) the parallel SDN. (Left) Alexa647 channel, (second column from the left) NBD channel, (third column from the left) overlay images, (right) line plot analyses along the white lines shown in the overlay images. c 3D Airyscan CLSM imaging of the parallel SDN. Green: NP-Alexa647, magenta: NBD-cycC6. Condition: [Ald-F(F)F] = 17.3 mM (0.80 wt%), [Phos-MecycC5] = 2.4 mM (0.15 wt%), [carboxymethoxylamine] = 20.8 mM (1.2 eq), [O-benzylhydroxylamine] = 69.2 mM (4.0 eq), [NP-Alexa647] = 4.0 µM, [NBD-cycC6] = 4.0 µM, 100 mM MES, pH 6.0, 30 °C.