Table 1.
List of experiments collected to perform the sequence specific assignment of apo-Nsp3b (A) and ADP-ribose bound Nsp3b (B). Main parameters used are reported
| Experiments | Time domain data size (points) | Spectral width (ppm) | ns | Delay time (s) | ||||
|---|---|---|---|---|---|---|---|---|
| t1 | t2 | t3 | F1 | F2 | F3 | |||
| A. apo-Nsp3b | ||||||||
| 1H-15N-HSQC | 256 | 2048 | 36.5 (15N) | 16.0 (1H) | 4 | 1.2 | ||
| 1H-15N best-TROSY | 256 | 2048 | 36.5 (15N) | 16.0 (1H) | 4 | 0.2 | ||
| Best-TROSY-HN(CO)CACB | 112 | 64 | 3072 | 75.3 (15N) | 32.2 (15N) | 13.9 (1H) | 40 | 0.25 |
| Best-TROSY-HNCACB | 112 | 64 | 3072 | 75.3 (13C) | 32.2 (15N) | 13.9 (1H) | 40 | 0.25 |
| Best-TROSY-HN(CA)CO | 104 | 64 | 3072 | 14.7 (13C) | 36.5 (15N) | 13.9 (1H) | 40 | 0.25 |
| Best-TROSY-HNCO | 104 | 64 | 3072 | 14.7 (13C) | 36.5 (15N) | 13.9 (1H) | 4 | 0.25 |
| 15N R1 | 10 | 128 | 2048 | 10.0 (1H) | 35.0 (15N) | 14.0 (1H) | 16 | 1.2 |
| 15N R2 | 10 | 128 | 2048 | 10.0 (1H) | 35.0 (15N) | 14.0 (1H) | 16 | 1.2 |
| 15N-NOE | 2 | 128 | 2048 | 10.0 (1H) | 35.0 (15N) | 14.0 (1H) | 16 | 3 |
| B. Nsp3b-ADP-ribose | ||||||||
| 1H-15N-HSQC | 256 | 2048 | 36.5 (15N) | 16.0 (1H) | 4 | 1.2 | ||
| 1H-15N best-TROSY | 256 | 2048 | 36.5 (15N) | 16.0 (1H) | 4 | 0.2 | ||
| Best-TROSY-HN(CO)CACB | 112 | 64 | 3072 | 75.3 (15N) | 32.2 (15N) | 13.9 (1H) | 48 | 0.25 |
| Best-TROSY-HNCACB | 112 | 64 | 3072 | 75.3 (13C) | 32.2 (15N) | 13.9 (1H) | 40 | 0.25 |
| Best-TROSY-HNCO | 104 | 64 | 3072 | 14.7 (13C) | 36.5 (15N) | 13.9 (1H) | 4 | 0.25 |
| 15N R1 | 10 | 128 | 2048 | 10.0 (1H) | 35.0 (15N) | 14.0 (1H) | 16 | 1.2 |
| 15N R2 | 10 | 128 | 2048 | 10.0 (1H) | 35.0 (15N) | 14.0 (1H) | 16 | 1.2 |
| 15N-NOE | 2 | 128 | 2048 | 10.0 (1H) | 35.0 (15N) | 14.0 (1H) | 16 | 3 |