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. 2020 Aug 4;16(8):e1008732. doi: 10.1371/journal.ppat.1008732

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© 2020 Caddy et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 1 — (A) Images of rotavirus-infected cells following electroporation of anti-VP6 MAb. Rotavirus-infected cells were stained with sheep polyclonal anti-rotavirus antibody and subsequently Alexa-Fluor 488-conjugated anti-sheep Ig. Scale bar 200μm (B) Fluorescent foci in (A) captured on a Nikon Eclipse Ti microscope were quantified by NIS analysis software. (C) Confocal images of a rotavirus-infected murine embryonic fibroblast cell 1 hour post infection in the presence of electroporated anti-VP6 Mab, scale bar 10μm. (D) Comparison of extracellular versus intracellular neutralisation for two anti-VP6 MAbs, 7D9 and 2C5. (E) Testing for intracellular neutralisation mediated by MAbs recognising VP4 (7A12 and 1A9).