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. 2020 Aug 4;16(8):e1008741. doi: 10.1371/journal.ppat.1008741

Fig 2. Galectin-3 binds to the surface of A. fumigatus, but does not affect fungal growth.

Fig 2

(A) Resting conidia, swollen conidia, and hyphae of red-fluorescent protein-expressing A. fumigatus strain Af293 (red), stained with biotinylated recombinant galectin-3 followed by fluorescently-labeled streptavidin (rGal-3, green), or (B) fluorescently-labeled streptavidin alone (Streptavidin only). Scale bar = 2 μm for resting and swollen conidia, and 5 μm for young hyphae. (C) Mean fluorescent intensity (MFI) of (A) and (B), normalized to swollen conidia. Results are the mean of 3 independent experiments. 2-way ANOVA with Sidak’s multiple comparison test. (D) The viability of A. fumigatus Af293 conidia grown for 24 hours in the presence of 850 μg/ml recombinant Galectin-3 (rGal-3) or 8 μg/ml amphotericin B (AmB). Results are the mean of 3 independent experiments. p values reported compared to untreated conidia; 1-way ANOVA with Sidak’s multiple comparison test.