Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Mus musculus) | Sp7(Osx) | NCBI Gene | RRID:MGI:2153568 | NCBI ID:170574 |
| Genetic reagent (Mus musculus) | Tg(Sp7-tTA,tetO-EGFP/cre)1Amc/J (Osx-cre) |
The Jackson Laboratories | Cat#006361 RRID:MGI:3689350 |
C57Bl/6 |
| Genetic reagent (Mus musculus) | B6.Cg-Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J (TdT) |
The Jackson Laboratories | RRID:MGI3813511 | C57Bl/6 |
| Genetic reagent (Mus musculus) | Sp7-creERT2/Ai9tdTomato (Osx-creERT2;TdT) |
PMID:31768488 | Cat#007909 RRID:MGI:4829803 |
Prof. Silva MJ (Washington University in St Louis) C57Bl/6 |
| Cell line (Mus musculus, C57Bl/6) | B16-F10 melanoma cell line | ATCC | RRID:CRL-6475-LUC2 | C57Bl/6 |
| Cell line (Mus musculus) | PyMT breast cancer cell line | PMID:27216180 | Prof. Weilbaecher KN (Washington University in St Louis) C57Bl/6 |
|
| Cell line (Mus musculus) | Immortalized Cancer Associated Fibroblast cell line | PMID:27264173 | Prof Longmore GD (Washington University in St Louis) | |
| Antibody | Rat anti-mouse CD45-APCeFluor780(clone 30-F11) | eBioscience | Cat #47-0451-82 RRID:AB_1548781 |
FACS (1:400) |
| Antibody | Rat anti-mouse CD45-PE Cy7 (clone 30-F11) | eBioscience | Cat #25-0451-82 RRID:AB_2734986 |
FACS (1:400) |
| Antibody | Rat anti-mouse CD11b-AlexaFluor700 (clone M1/70) | eBioscience | Cat#56-0112-82 RRID:AB_657585 |
FACS (1:400) |
| Antibody | Rat anti-mouse Gr1(Ly6G)-FITC (clone RB6-8C5) | Miltenyi Biotec | Cat#130-102-837 RRID:AB_2659858 |
FACS (1:100) |
| Antibody | Rat anti-mouse F4/80 PerCP Cy5.5 (clone BM8) | Biolegend | Cat#123126 RRID:AB_10802654 |
FACS (1:400) |
| Antibody | Hamster anti-mouse CD3e-PE (clone 145–2 C11) | eBioscience | Cat#A14714 RRID:AB_2534230 |
FACS (1:200) |
| Antibody | Rat anti-mouse CD4-APC (clone RM4-5) | BD Biosciences | Cat#553051 RRID:AB_398528 |
FACS (1:200) |
| Antibody | Rat anti-mouse CD8a-FITC (clone 53–6.7) | BD Biosciences | Cat#561966 RRID:AB_10896291 |
FACS (1:200) |
| Antibody | Mouse anti-mouse NK1.1 (clone PK136) | Biolegend | Cat#108722 RRID:AB_2132712 |
FACS (1:200) |
| Antibody | Rat anti-mouse CD45R (B220)-Biotin (clone RA3-6B2) | eBioscience | Cat#13-0452-82 RRID:AB_466449 |
FACS (0.4 ul/sample) |
| Antibody | Hamster anti-mouse CD3e-Biotin (clone 145–2 C11) | eBioscience | Cat#13-0031-82 RRID:AB_466319 |
FACS (0.4 ul/sample) |
| Antibody | Rat anti-mouse Ter119 (clone TER119) | eBioscience | Cat# 13-5921-82 RRID:AB_466797 |
FACS (0.4 ul/sample) |
| Antibody | Rat anti-mouse Ly-6G/Ly-6C Biotin (clone RB6-8C5), | eBioscience | Cat#13-5931-82 RRID:AB_466800 |
FACS (0.2 ul/sample) |
| Antibody | Rat anti-mouse CD41Biotin (clone MWReg30) | eBioscience | Cat#13-0411-82 RRID:AB_763484 |
FACS (1 ul/sample) |
| Antibody | Streptavidin-BV510 | BD Biosciences | Cat#563261 | FACS (0.5 ul/sample) |
| Antibody | Rat anti-mouse Sca-1 BV711 (clone D7) | BD Biosciences | Cat#563992 RRID:AB_2738529 |
FACS (0.25 ul/sample) |
| Antibody | Rat anti-mouse c-kit(CD117)-APCeFluor780 (clone ACK2) | eBioscience | Cat#47-1172-82 RRID:AB_1582226 |
FACS (1 ul/sample) |
| Antibody | Rat anti-mouse CD16/32-BUV395 (clone 2.4G2) | BD Biosciences | Cat#740217 RRID:AB_2739965 |
FACS (0.5 ul/sample) |
| Antibody | Rat anti-mouse CD34-FITC (clone RAM34) | eBioscience | Cat#11-0341-82 RRID:AB_465021 |
FACS (4 ul/sample) |
| Antibody | Rat anti-mouse CD150(SLAM)-BV421 (clone TC15-12F12.2) | Biolegend | Cat#115943 RRID:AB_2650881 |
FACS (1 ul/sample) |
| Antibody | Hamster anti-mouse CD48-PE Cy7 (clone HM48-1) | eBioscience | Cat#25-0481-80 RRID:AB_1724087 |
FACS (0.15 ul/sample) |
| Antibody | Rat anti-mouse CD135(Flt3)-APC (clone A2F10) | eBioscience | Cat#17-1351-82 RRID:AB_10717261 |
FACS (2 ul/sample) |
| Antibody | Rat anti-mouse CD45 (clone 30-F11) | Invitrogen | Cat#14-0451-82 RRID:AB_467251 |
IF, IHC (1:200) |
| Antibody | Rabbit anti-mouse RFP/TdT | Rockland | Cat#600-401-379 RRID:AB_2209751 |
IF,IHC (1:500) |
| Antibody | Goat-anti-Rat IgG-Biotin | Thermo Fisher | Cat#31830 RRID:AB_228355 |
IF, IHC (1:500) |
| Antibody | Streptavidin-HRP | Bio-Rad | Cat#STAR5B | IF, IHC (1:500) |
| Antibody | Goat-anti-rabbit Alexa-fluor647 | Thermo Fisher | Cat#A21244 RRID:AB_2535812 |
IF (4 ug/ml) |
| Antibody | Rabbit anti-mouse Sp7/Osx | Abcam | Cat#ab227820 | IF (1:500) |
| Antibody | Goat anti-rabbit AlexaFluor488 | Abcam | Cat#ab150077 RRID:AB_2630356 |
IF (1:1000) |
| Sequence-based reagent | Cyclophilin | This paper | PCR primers | 5’-AGC ATA CAG GTC CTG GCA TC-3’ and 5’-TTC ACC TTC CCA AAG ACC AC-3’ |
| Sequence-based reagent | Sp7(Osx) | This paper | PCR primers | 5’-AAG GGT GGG TAG TCA TTT GCA-3’ and 5’-CCC TTC TCA AGC ACC AAT GG-3’ |
| Sequence-based reagent | S100A4(Fsp-1) | This paper | PCR primers | 5’-TGA GCA ACT TGG ACA GCA ACA-3’ and 5’-TTC CGG GGT TCC TTA TCT GGG-3’ |
| Sequence-based reagent | Acta2(α-SMA) | This paper | PCR primers | 5’-GTC CCA GAC ATC AGG GAG TAA-3’ and 5’-TCG GAT ACT TCA GCG TCA GGA-3’ |
| Sequence-based reagent | Col1a1 | This paper | PCR primers | 5’-GGC CTT GGA GGA AAC TTT GC-3’ and 5’-GGG ACC CAT TGG ACC TGA AC-3’ |
| Sequence-based reagent | Bglap(Ocn) | This paper | PCR primers | 5’-GGA CTG AGG CTC TGT GAG GT-3’ and 5’-CAG ACA CCA TGA GGA CCA TC-3’ |
| Sequence-based reagent | Runx2 | This paper | PCR primers | 5’-GTT ATG AAA AAC CAA GTA GCC AGG-3’ and 5’-GTA ATC TGA CTC TGT CCT TGT GGA-3’ |
| Sequence-based reagent | BSP | This paper | PCR primers | 5’-AGG ACT AGG GGT CAA ACA C-3’ and 5’-AGT AGC GTG GCC GGT ACT TA-3’ |
| Sequence-based reagent | TNAP | This paper | PCR primers | 5’-GGG GAC ATG CAG TAT GAG TT-3’ and 5’-GGC CTG GTA GTT GTT GTG AG-3’ |
| Commercial assay or kit | TSA FITC System | Perkin Elmer | Cat#NEL701A001KT | |
| Commercial assay or kit | BOND Intense R detection kit | Leica Biosystem | Cat#DS9263 | |
| Commercial assay or kit | BOND Polymer Refine Red detection kit | Leica Biosystem | Cat#DS9390 | |
| Commercial assay or kit | Avidin/Biotin Blocking kit | Vector Labs | Cat#SP2001 RRID:AB_2336231 |
|
| Software, algorithm | STAR | STAR | RRID:SCR_015899 | Version 2.0.4b |
| Software, algorithm | Subread | Subread | RRID:SCR_009803 | Version 1.4.5 |
| Software, algorithm | R | R | RRID:SCR_001905 | Version 3.4.1 |
| Software, algorithm | EdgeR | EdgeR | RRID:SCR_012802 | Version 3.20.2 |
| Software, algorithm | LIMMA | LIMMA | RRID:SCR_010943 | Version 3.34.4 |
| Software, algorithm | GAGE | GAGE | RRID:SCR_017067 | Version 2.28.0 |
| Other | VECTASHIELD Mounting Medium with DAPI | Vector Labs | Cat#H1200 RRID:AB_2336790 |
one drop |
| Other | ProLong Gold with DAPI | Invitrogen | Cat#P10144 | one drop |