Table 2.
Sample ID | Origin | Date of sampling | WWTP | Nested RT-PCR | Real-time RT-(q)PCR (c.g./L) |
---|---|---|---|---|---|
3285 | Milan | 24/10/2019 | A | − | − |
3287 | Milan | 25/11/2019 | A | − | − |
3289 | Milan | 18/12/2019 | A | + | 4.1 × 103 |
3290 | Milan | 18/12/2019 | A2 | − | 8.7 × 102 |
3238 | Milan | 20/12/2019 | B | + | 1.2 × 103 |
3291 | Milan | 29/01/2020 | A | + | 2.3 × 103 |
3292 | Milan | 29/01/2020 | A2 | − | 2.2 × 103 |
3244 | Milan | 03/02/2020 | B | − | 6.1 × 102 |
3231 | Milan | 12/02/2020 | A | − | 1.6 × 103 |
3239 | Milan | 12/02/2020 | B | − | 2.8 × 103 |
3232 | Milan | 19/02/2020 | A | + | − |
3240 | Milan | 19/02/2020 | B | − | 2.6 × 103 |
3241 | Milan | 23/02/2020 | B | + | 1.5 × 103 |
3233 ⁎ | Milan | 24/02/2020 | A | + | 9.2 × 102 |
3230 | Milan | 25/02/2020 | A | + | 4.8 × 102 |
3237 | Milan | 25/02/2020 | A2 | − | 1.4 × 103 |
3234 | Milan | 26/02/2020 | A | + | 3.7 × 103 |
3242 | Milan | 26/02/2020 | B | − | 1.7 × 103 |
3235 | Milan | 28/02/2020 | A | − | − |
3243 ⁎ | Milan | 28/02/2020 | B | + | 1.3 × 103 |
3144 | Turin | 09/10/2019 | C | − | − |
3145 | Turin | 09/10/2019 | C | − | − |
3321 | Turin | 06/11/2019 | C | − | − |
3323 | Turin | 06/11/2019 | D | − | − |
3325 | Turin | 20/11/2019 | C | − | − |
3329 | Turin | 04/12/2019 | C | − | − |
3331 | Turin | 04/12/2019 | D | − | − |
3333 | Turin | 18/12/2019 | C | + | − |
3335 | Turin | 18/12/2019 | D | + | 1.2 × 103 |
3337 | Turin | 14/01/2020 | C | + | 7.4 × 102 |
3339 | Turin | 15/01/2020 | D | + | 1.2 × 103 |
3341 | Turin | 28/01/2020 | D | + | 5.6 × 102 |
3343 | Turin | 29/01/2020 | C | − | 6.0 × 102 |
3345 | Turin | 11/02/2020 | D | − | 4.7 × 102 |
3347 | Turin | 25/02/2020 | D | + | 2.9 × 102 |
3349 | Turin | 26/02/2020 | C | + | 5.6 × 104 |
3374 | Bologna | 21/11/2019 | E | − | − |
3375 | Bologna | 10/12/2019 | E | − | 2.9 × 104 |
3376 | Bologna | 29/01/2020 | E | + | 3.3 × 104 |
3377 | Bologna | 19/02/2020 | E | + | − |
Highlighted in bold are the first occurrences of SARS-CoV-2 in each of the urban areas included in the study. ‘A2’ represents a second branch of the ‘A' wastewater treatment plant. Samples below 5.9 × 103 g.c./L (LOQ) should be considered as estimated counts.
Samples detected as positive in a previous study (La Rosa et al., 2020) and confirmed as such by repeating both the extraction and the molecular analysis.