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. 2020 Apr 16;9(8):e015748. doi: 10.1161/JAHA.119.015748

Figure 6. Western blot analysis of primary cardiomyocytes treated with B7‐33.

Figure 6

In primary cardiomyocytes, increased phosphorylation of extracellular signal‐regulated kinase (Erk) 1/2 (p‐Erk 1/2) in cells treated with B7‐33, reaching significance at 100 nmol/L (A); reduced expression of ASC (apoptosis‐associated speck‐like protein containing a caspase recruitment domain) in cells 6 hours post simulated ischemia reoxygenation (SIRO), on treatment with B7‐33 (B); and reduced expression of GRP78 in cells 24 hours post SIRO, on treatment with B7‐33 (C) (for A through C, n=3 experiments per group). Western blots are quantified and expressed as arbitrary densitometric units (A.D.U). For A through C, 1‐way ANOVA was used to determine significance, and if P<0.05, Holm‐Sidak test was used for post hoc analysis. *P<0.05. C indicates control.