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. 2020 Apr 16;9(8):e015748. doi: 10.1161/JAHA.119.015748

Figure 7. Western blot analysis of tunicamycin‐induced endoplasmic reticulum (ER) stress in protein lysates from primary cardiomyocytes.

Figure 7

Exposure of primary cardiomyocytes to tunicamycin (TUN) for 24 hours significantly reduces phosphorylation of extracellular signal‐regulated kinase (Erk) 1/2 (p‐Erk 1/2) at both 2.5 and 7.5 μg/mL concentrations of TUN (A); and significantly increases expression of GRP78 at both doses of TUN (B). B7‐33 (100 nmol/L) significantly lowers GRP78, compared with control (C), on coincubation with 2.5 μg/mL TUN (for A and B, n=4–6 experiments/group). Western blots are quantified and expressed as arbitrary densitometric units with respect to (A.D.U WRT) the expression of GAPDH. For A and B, 1‐way ANOVA was used for significance testing, and if P<0.05, Holm‐Sidak test was used for post hoc analysis. *P<0.05, **P<0.01, ****P<0.0001.