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. 2020 Apr 16;9(8):e015748. doi: 10.1161/JAHA.119.015748

Figure 8. Treatment of primary cardiomyocytes with tunicamycin (TUN) with/without PD98059 and/or B7‐33.

Figure 8

A, Representative Western blots of primary cardiomyocytes treated with tunicamycin (TUN) with/without PD98059 and/or B7‐33. B, Exposure to PD98059 (50 μmol/L) significantly lowers phosphorylation of extracellular signal‐regulated kinase (Erk) 1/2 (p‐Erk 1/2) 24 hours post incubation in primary cardiomyocytes treated with TUN with/without B7‐33. No significant difference between p‐Erk 1/2 levels was found between TUN and TUN+B7‐33 groups. C, Coincubating TUN with PD98059 abrogates the reduction in GRP78 observed when treated with B7‐33 (for B and C, n=3 experiments/group). Western blots are quantified and expressed as arbitrary densitometric units with respect to (A.D.U WRT) the expression of GAPDH. For B and C, 1‐way ANOVA was used for significance testing, and if P<0.05, Holm‐Sidak test was used for post hoc analysis. *P<0.05.