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. 2020 Apr 19;9(8):e014757. doi: 10.1161/JAHA.119.014757

Figure 1. Spermidine oral administration does not alter mouse feed intake and body weight gain.

Figure 1

C57BL/6 male mice (8–10 weeks old) were administered 3 mmol/L spermidine in drinking water or normal drinking water as vehicle before PPE infusion to day 14 after PPE infusion. A, Plasma spermidine concentrations were measured by LC/MS. Unpaired t test, *P<0.05 vs vehicle group. n=4 to 5 mice/group. B, No difference in food intake in 2 treatment groups during whole experimental process. Two‐way ANOVA with the Bonferroni correction, P=0.879 vs vehicle group. C, No difference in body weight at the baseline level (P=0.665) and 14 days (P=0.857) following treatment with vehicle (n=15) or spermidine (n=13). Two‐way ANOVA. NS for nonsignificance, vs vehicle group. All data are means±SD. LC/MS indicates liquid chromatography‐mass spectrometry; PPE, porcine pancreatic elastase; and SPD, spermidine.