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. 2020 Apr 19;9(8):e014757. doi: 10.1161/JAHA.119.014757

Figure 2. Spermidine treatment prevents development of experimental AAA.

Figure 2

External aortic diameter was measured by computer software under laparotomy. A, Representative aortic images for the baseline (day 0) and 14 days after PPE infusion. B, Mean and SD of aortic diameter (mm) in mice treated with vehicle (n=16) or SPD (n=18) at day 0 and day 14. Unpaired t test, **P<0.01 vs vehicle group. C, AAA incidence. Fisher exact test, P=0.046 vs vehicle group. D and E, Representative images as well as mean±SD of destruction scores for EVG (P=0.004), Masson's (P=0.017), and SMC α actin staining (P=0.001). Scale bar=100 and 500 μm. Medial elastin and SMC destruction were graded as I (mild) to IV (severe). Nonparametric Mann‐Whitney test, **P<0.01 and *P<0.05 vs vehicle group, n=7 to 8 mice/group. AAA indicates abdominal aortic aneurysm; EVG, Verhoeff's Van Gieson; PPE, porcine pancreatic elastase; SMC, smooth muscle cell; and SPD, spermidine.