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. 2020 Apr 19;9(8):e014757. doi: 10.1161/JAHA.119.014757

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© 2020 The Authors. Published on behalf of the American Heart Association, Inc., by Wiley.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Whole blood samples collected from heart 14 days after PPE infusion were stained with monoclonal antibodies against CD45, CD11b, Ly‐6C, and Ly‐6G. Inflammatory monocytes (CD11b⁺Ly‐6C^high) and neutrophils (CD11b⁺Ly‐6G⁺) in blood samples in SPD or vehicle group were analyzed by flow cytometry. Data presented as mean and SD of the percentages of inflammatory monocytes (P=0.046) and neutrophils (P=0.043) in total leukocytes gated by forward scatter and CD45. Unpaired t test with Welch's correction, *P<0.05 vs vehicle‐treated group, n=4 to 6 mice/group. PPE indicates porcine pancreatic elastase; and SPD, spermidine.