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. 2020 Apr 2;9(7):e014146. doi: 10.1161/JAHA.119.014146

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© 2020 The Authors. Published on behalf of the American Heart Association, Inc., by Wiley.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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Real‐time reverse transcription‐polymerase chain reaction (RT‐PCR) analysis of expression of ANRIL, TMEM100, and TMEM106B in endothelial cells (ECs) transfected with different siRNA.

A, Relative expression of ANRIL in EA.hy926 cells transiently co‐transfected with different combinations of small interfering (si)NC (negative control siRNA), siANRIL, siTMEM100, or siTMEM106B was quantified by quantitative RT‐PCR (qRT‐PCR) (n=6 samples/group; 3 wells/experiment with each experiment repeated again). B, Relative expression of ANRIL in human coronary artery endothelial cells transiently co‐transfected with different combinations of siNC, siANRIL, siTMEM100, or siTMEM106B (n=6 samples/group; 3 wells/experiment with each experiment repeated again) was quantified by qRT‐PCR. C, Relative expression of TMEM100 in EA.hy926 cells transiently co‐transfected with different combinations of siNC, siANRIL, siTMEM100, or siTMEM106B (n=9 samples/group; 3 wells/experiment with each experiment repeated twice again) was quantified by qRT‐PCR. D, Relative expression of TMEM100 in human coronary artery endothelial cells transiently co‐transfected with different combinations of siNC, siANRIL, siTMEM100, or siTMEM106B (n=9 samples/group; 3 wells/experiment with each experiment repeated twice again) was quantified by qRT‐PCR. E, Relative expression of TMEM106B in EA.hy926 cells transiently co‐transfected with different combinations of siNC, siANRIL, siTMEM100, or siTMEM106B (n=9 samples/group; 3 wells/experiment with each experiment repeated twice again) was quantified by qRT‐PCR. F, Relative expression of TMEM106B in human coronary artery endothelial cells transiently co‐transfected with different combinations of siNC, siANRIL, siTMEM100, or siTMEM106B (n=9 samples/group; 3 wells/experiment with each experiment repeated twice again) was quantified by qRT‐PCR. Statistical analysis was performed using unpaired, 2‐tailed Student t‐tests. Si indicates small interfering.