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. 2020 Apr 2;9(7):e014146. doi: 10.1161/JAHA.119.014146

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© 2020 The Authors. Published on behalf of the American Heart Association, Inc., by Wiley.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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Overexpression of TMEM106B reverses the effects of ANRIL overexpression on monocyte adhesion to endothelial cells and transendothelial migration of monocytes.

A, Relative expression of ANRIL in a scale of log₁₀ in EA.hy926 cells transiently co‐transfected with different combinations of pcDNA3.1, pcDNA3.1‐ANRIL, or pcDNA3.1‐TMEM106B (n=9 samples/group; 3 wells/experiment with each experiment repeated twice again). B, Relative expression of ANRIL in human coronary artery endothelial cells transiently co‐transfected with different combinations of pcDNA3.1, pcDNA3.1‐ANRIL, or pcDNA3.1‐TMEM106B (n=9 samples/group; 3 wells/experiment with each experiment repeated twice again). C, Relative expression of TMEM106B in EA.hy926 transiently co‐transfected with different combinations of pcDNA3.1, pcDNA3.1‐ANRIL, or pcDNA3.1‐TMEM106B (n=9 samples/group; 3 wells/experiment with each experiment repeated twice again). D, Relative expression of TMEM106B in human coronary artery endothelial cells transiently co‐transfected with different combinations of pcDNA3.1, pcDNA3.1‐ANRIL, or pcDNA3.1‐TMEM106B (n=9 samples/group; 3 wells/experiment with each experiment repeated twice again). E, Relative adhesion of monocytes to EA.hy926 cells transiently co‐transfected with different combinations of pcDNA3.1, pcDNA3.1‐ANRIL, or pcDNA3.1‐TMEM106B (n=9 samples/group; 3 wells/experiment with each experiment repeated twice again). F, Relative adhesion of monocytes to human coronary artery endothelial cells transiently co‐transfected with different combinations of pcDNA3.1, pcDNA3.1‐ANRIL, or pcDNA3.1‐TMEM106B (n=9 samples/group; 3 wells/experiment with each experiment repeated twice again). G, Relative transmigration of monocytes across a layer of EA.hy926 cells transiently co‐transfected with different combinations of pcDNA3.1, pcDNA3.1‐ANRIL, or pcDNA3.1‐TMEM106B was quantified (n=9 samples/group; 3 wells/experiment with each experiment repeated twice again). H, Relative transmigration of monocytes across human coronary artery endothelial cells transiently co‐transfected with different combinations of pcDNA3.1, pcDNA3.1‐ANRIL, or pcDNA3.1‐TMEM106B (n=9 samples/group; 3 wells/experiment with each experiment repeated twice again). Statistical analysis was performed using unpaired, 2‐tailed Student t‐tests.