. 2020 Aug 16;2:100007. doi: 10.1016/j.talo.2020.100007

Table 4.

Respiratory syncytial virus biosensors data.

Detection technique	Substrate	Immobilized material / Analyte	Monitored compound	Working Range	LOD	Sample	Ref.
Optical – SPR	Gold chip	RSV oligonucleotide probe / RSV PCR product	Label-free detection	1.00 nM – 1.00 µM	3.00 nM	Throat swab specimens	[185]
Optical – Microarray scanner	Gold-plated tungsten filaments	RSV DNA hairpin structures (molecular beacon style)/ RNA from cell culture	Label-free detection	Not determined	11.9 PFU	RNA target extrated from cell culture	[193]
Electrochemical – Amperometric through SPE	Polystyrene slide	Anti-RSV/ RSV antigen (sandwich ELISA-like assay)	TMB	Not determined	1:5000 (RSV Ag dilution)	Respiratory secretion clinical samples	[194]
Electrochemical – Amperometric	Gold chip	aMB with SA-HRP/ RSV DNA	TMB	100 pM – 100 nM	11.0 pM	RSV DNA in 10% human serum	[195]
		aMB with SA-HRP/ RSV miRNA let-7a		20.0 pM – 100 nM	3.40 pM
Optical – Absorbance	Plate/Gold nanoparticle layer	Anti-RSV / RSV antigen	TMB	0.050 – 30.0 pg.mL⁻¹	0.010 pg.mL⁻¹	Human serum from healthy donor	[197]

aMB = Allosteric molecular beacons; SA-HRP = Streptavidin aptamer-horseradish peroxidase; TMB = 3,3,5,5-Tetramethylbenzidine;.