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. 2020 Jun 10;9(11):e016099. doi: 10.1161/JAHA.120.016099

Figure 4. SH3 domains of Nck (noncatalytic region of tyrosine kinase) 1 are essential for flow‐induced endothelial permeability.

Figure 4

A, Schematic showing the domain structure of Nck1 and Nck2 and 2 chimera of Nck1 SH2/SH3 and Nck1 SH3/Nck2 SH2. B, Western blot analysis showing comparable Nck1/2 re‐expression following transduction of constructs in (A) in Nck1/2 double knockout (DKO) cells. C and D, Permeability of endothelial cells after oscillatory shear stress (OSS) as assessed by streptavidin‐biotinylated gelatin trapping assay. Data are analyzed by 2‐way ANOVA and Bonferroni posttest (*P<0.05; ns, nonsignificant, from n=4). E, Schematic diagram suggesting that Nck1/2 bind to PECAM‐1 (platelet endothelial cell adhesion molecule‐1) in response to shear stress, but only Nck1 through its SH3 domains regulates the permeability.