Figure 8. Tranilast (TR) predominantly targeted the NLRP3 (nucleotide‐binding oligomerization domain, leucine‐rich repeat–containing receptor family pyrin domain‐containing 3) inflammasome in macrophages.

The frozen section of the aortic sinuses from mice treated with TR or vehicle containing dimethyl sulfoxide (DM) were analyzed by dual immunofluorescence staining. Double immunostaining was conducted with the antibodies against α–smooth muscle actin (α‐SMA) and apoptosis‐associated speck‐like protein containing a caspase recruitment domain (ASC) (A), Mac3 and ASC (A), CD68 and interleukin (IL)‐1β (B).