2.

单克隆抗体特征

Characteristic features of the anti-CII monoclonal antibodies

Antibody	Epitope	Epitope sequence of the a-chain	Isotypes	V genes
All epitopes are in native triple helical form. G: Glycine; P: Proline or Hydroxyproline; E: Glutamic acid; R: Arginine; K: Lysine; H: Histidine; F: Phenylalanine; A: Alanine; L: Leucine; T: Threonine; Y: Tyrosine. The C1 epitope consists of 3 distinct epitopes（C1Ⅰ; C1Ⅱ; C1Ⅲ）. CIIC1 binds the shortest part of the epitope that is critical for binding of all C1 specific antibodies.
CIIC1	C1Ib	359 - 363:ARGLT	IgG2aκ	J558/Vk21C
M2139	J1	551 - 564: GERGAAGIAGPK	IgG2bκ	J5582c/Vk21G
CIIC2	D3	687 - 698: RGAQGPPGATGF	IgG2bκ	X24/Vk21E
UL1	U1	494 - 504: GLVGPRGERGF	IgG2bκ	not done