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. 2020 Aug 12;12:7117–7130. doi: 10.2147/CMAR.S253152

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© 2020 Hui et al.

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CircNHSL1 knockdown inhibited migration, invasion and glutaminolysis of GC cells. (A and B) Relative levels of circNHSL1 and NHSL1 mRNA were tested in HGC-27 and AGS cells treated with or without RNase R. (C and D) The cellular localization of circNHSL1 in HGC-27 and AGS cells was analyzed by Subcellular fractionation. (E) The levels of circNHSL1 and NHSL1were detected in HGC-27 and AGS cells transfected with si-NC and si-circNHSL1. (F and G) Transwell migration assay was carried out to measure the capacity of migration in transfected HGC-27 and AGS cells. (H and I) Transwell invasion assay was applied to assess the ability of invasion in transfected HGC-27 and AGS cells. (J–L) Relative levels of glutamine, glutamate and α-KG were assessed in transfected HGC-27 and AGS cells. (M and N) Protein levels of ASCT2 and GLS1 were examined in transfected HGC-27 and AGS cells. *P <0.05.