Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Aug 12;12:7117–7130. doi: 10.2147/CMAR.S253152

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 Hui et al.

This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).

PMC Copyright notice

YWHAZ acted as the target of miR-149-5p. (A) Starbase3.0 software was conducted to predict the binding sequences between miR-149-5p and YWHAZ-3ʹUTR. (B and C) The effects of miR-149-5p overexpression on luciferase activity of WT-YWHAZ and MUT-YWHAZ reporters in HGC-27 and AGS cells were measured by a dual-luciferase reporter assay. (D) MiR-149-5p level was detected in HGC-27 and AGS cells transfected with miR-NC and miR-149-5p mimic. (E and F) YWHAZ level was measured in HGC-27 and AGS cells transfected with miR-NC and miR-149-5p mimic. (G and H) YWHAZ protein level was detected in GC tissues, GC cell lines (HGC-27 and AGS cells) and respective control groups. (I and J) YWHAZ level was measured in HGC-27 and AGS cells transfected with pc-NC and pc-YWHAZ. (K and L) YWHAZ level was detected in HGC-27 and AGS cells treated with miR-NC, miR-149-5p mimic, miR-149-5p mimic +pc-NC and miR-149-5p mimic + pc-YWHAZ. (M and N) Capacities of migration and invasion were analyzed in treated HGC-27 and AGS cells. *P <0.05.