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. 2020 Aug 11;13:7973–7984. doi: 10.2147/OTT.S264108

Figure 2.

Figure 2

Down-regulation of USP8 inhibits proliferation of gastric cancer cells. (A) Expression levels of USP8 and HER-3 in six gastric cancer lines as indicated, *P<0.05, **P<0.01. (B) Chemical structure of USP8 inhibitor (DUBs-IN-2). (C) The proliferation rates of cells were evaluated with indicated treatment. (D) Immunoblot analysis to confirm knockdown of USP8 using si-USP8 (si-USP8 sequence #1 and si-USP8 sequence #2) and gene glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as a control to verify equal loading of protein in NCI-N87, MKN-45, AGS and MGC-803 cell lines. (E) Clone formation experiments were performed on MGC-803 (USP8i, 2000 nmol/L and si-USP8 sequence #1), MKN-45 (USP8i, 500 nmol/L and si-USP8 sequence #1) and AGS (USP8i, 500 nmol/L and si-USP8 sequence #1) cells, *P<0.05, **P<0.01, ***P<0.001.

Abbreviations: NS, no statistical significance; USP8i, USP8 inhibitor.