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. Author manuscript; available in PMC: 2021 Aug 5.
Published in final edited form as: J Am Chem Soc. 2020 Jul 24;142(31):13372–13383. doi: 10.1021/jacs.9b11950

Figure 3.

Figure 3.

Representative plots of model-free PLIMSTEX data for the titration of 4 μM CP-Ser with Ca2+ ions. Data for S100A8(C42S) (A-D) and S100A9(C3S) (E-H) are shown. Peptide sequences and associated charge states are: (A) HAVYRDDL (S100A8(C42S) 27–34 (+2)), (B) TESPQYIRKKGADVW (S100A8(C42S) 40–54 (+2)), (C) FKELDINTDGAVNF (S100A8(C24S) 55–68 (+2)), (D) VIKMGVAAHKKSHEESHKE (S100A8(C42S) 75–93 (+3)), (E) SVKLGHPDTLNQGEF (S100A9(C3S) 23–37 (+2)), (F) FLKKENKNEKVIE (S100A9(C3S) 48–60 (+2)),(G)LDTNADKQLSFEE(S100A9(C3S)66–78(+2)), and(H) TWASHEKMHEGDEGPGHHHKPGLGEGTP (S100A9(C3S) 87–114 (+3), respectively. PLIMSTEX data for peptides from the EF-hands (A, C, E, and G) and other regions (B, D, F, H) are shown.