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. Author manuscript; available in PMC: 2021 Aug 5.
Published in final edited form as: J Am Chem Soc. 2020 Jul 24;142(31):13372–13383. doi: 10.1021/jacs.9b11950

Figure 4.

Figure 4.

PLIMSTEX data for the titration of 4 μM CP-Ser with Ca2+ ions fit to the NLLS algorithm using a 1:4 sequential binding model with no consideration of tetramerization. The data were acquired for four EF-hand peptides in the following charge states: HAVYRDDL (S100A8(C42S) 27–34 (+2)) (blue circles), FKELDINTDGAVNF (S100A8(C42S) 55–68 (+2)) (green squares), SVKLGHPDTLNQGEF (S100A9(C3S) 23–37 (+2)) (red diamonds), LDTNADKQLSFEE (S100A9(C3S) 66–78 (+2)) (black triangles), respectively.