Statistical significance was determined by two-way ANOVA with repeated measures, and defined as p ≤ 0.05.
A) At 16 months of age, MAP was similar in previously-pregnant and virgin controls. MAP in previously-pregnant HAF rats was not different than virgin controls but was higher than previously-pregnant controls. MAP was higher in virgin HAF rats than all controls or previously pregnant HAF rats.
B) MAP at 10 months of age was higher in virgin and previously-pregnant HAF rats than in virgin or previously-pregnant controls. Pregnancy had no effect on MAP in either controls or HAF rats.
C) Comparison of MAP at 10 months and 16 months of age in virgin and previously pregnant HAF rats. MAP was not different in previously pregnant HAF rats at 16 months of age compared to either virgins or previously pregnant HAF rats at 10 months of age. However, MAP increased in virgin HAF rats between 10 months and 16 months of age.
D) Comparison of systolic blood pressure (SBP) at 10 months and 16 months of age in virgin and previously-pregnant HAF rats. SBP was not different in previously-pregnant HAF rats at 16 months of age compared to either virgins or previously-pregnant HAF rats at 10 months of age. However, SBP increased in virgin HAF rats between 10 months and 16 months of age, and there was significant interaction with age and parity.
E) Comparison of DBP at 10 months and 16 months of age in virgin and previously-pregnant HAF rats. DBP was not different in vigin or previously-pregnant control rats at 10 or 16 months of age. There was no effect of age or parity and there were no interactions.