Figure 3.
Optimization for SAHH-coupled ThioGlo3 assay. A) NTMT1 titration and time-course in 384-well format. B) Assay performance (Z’-factor) under different concentrations of NTMT1 in 384-well format. C) NTMT1 titration and time-course in 1536-well format. D) Assay performance (Z’-factor) under different concentrations of NTMT1 at room temperature and 37°C in 1536-well format.