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. 2020 Aug 9;2(8):489–505. doi: 10.1096/fba.2020-00013

FIGURE 4.

FIGURE 4

Electrophoretic mobility of human and Pf CaM. Samples of human and Pf CaM were run in the presence and absence of Ca2+ using (A) native (Imidazole/HEPES) PAGE, or (B) SDS‐PAGE. A, Migration pattern of 10 µmol/L (1.7 µg) CaM in the presence of 1 mmol/L EGTA or CaCl2, and 20 µmol/L melittin. B, Migration pattern of CaM after incubation with 5 mmol/L EGTA or CaCl2 using SDS‐PAGE (15% acrylamide gel)