FIGURE 9.
Pf CaM assays with MMV396736. A, The effect of MMV396736 on target enzyme activation was tested on CaN, and an IC50 = 6.2 ± 1.5 μmol/L was calculated from fitting to dose‐response function. B, MMV396736 binding to dansyl‐labeled Pf CaM was followed using dansyl fluorescence. Data were fitted to a hyperbolic function, obtaining K d = 2.4 ± 0.4 μmol/L. C, MMV396736 was titrated with Pf CaM using ITC. Data were fitted with the one set of sites model, yielding n = 7.4 ± 0.1, and K d = 2.5 ± 0.2 μmol/L. The chemical structure of the compound MMV396736 is also shown. Image was taken from PubChem (https://pubchem.ncbi.nlm.nih.gov). It is positioned in well G8, plate C (drug‐like molecules) of the Malaria Box (https://www.mmv.org/sites/default/files/uploads/docs/malariabox/PlateC.html)