TABLE 1.
Thermodynamic parameters for the thermal denaturation of human and Pf CaM determined by DSC, CD, and DSF. Note that heating rate may differ (as it is 1°C/min for DSC and CD while 5°C/min for DSF
| Method | Parameter | Human apoCaM | Human CaCaM | Pf apoCaM | Pf CaCaM |
|---|---|---|---|---|---|
| DSC | T m1 (°C) | 46.7 ± 0.1 | 91.6 ± 0.1 | 54.7 ± 0.2 | 92.6 ± 0.1 |
| ΔH1 (kcal/mol) | 6.5 ± 0.4 | 37.7 ± 0.6 | 41.2 ± 1.2 | 47.8 ± 0.3 | |
| ΔHv1 (kcal/mol) | 57.2 ± 1.1 | 49.0 ± 0.7 | 48.1 ± 0.4 | 44.5 ± 0.3 | |
| T m2 (°C) | 59.2 ± 0.1 | 109.8 ± 0.1 | 64.3 ± 0.1 | 125.9 ± 0.2 | |
| ΔH2 (kcal/mol) | 44.4 ± 0.3 | 73.9 ± 0.5 | 33.4 ± 1.2 | 97.9 ± 2.2 | |
| ΔHv2 (kcal/mol) | 44.5 ± 0.2 | 78.5 ± 0.5 | 53.5 ± 0.6 | 65.7 ± 0.8 | |
| CD | T m (°C) | 50.8 ± 0.1 | 54.4 ± 0.2 | ||
| T m (°C) a | 47.1 ± 0.1 | 51.2 ± 0.1 | |||
| DSF | T m (°C) | 38.9 ± 0.5 | 51.2 ± 0.6 |
a
In phosphate buffer of low ionic strength.