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Time-dependent inhibition study of compounds 1–3. All experiments were run with duplicate (n=2), except 10 min of pre-incubation (n=3). (A), (C) and (E) Concentration–response plots for different pre-incubation times fitted to Morrison’s quadratic equation for compounds 1, 2 and 3, respectively ; (B), (D) and (F) Nonlinear regression of Morrison Ki, app values with pre-incubation time for compounds 1, 2 and 3, respectively.
