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. 2020 Aug 10;12:7087–7095. doi: 10.2147/CMAR.S265775

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© 2020 Zheng et al.

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MMP14 and MMP16 are direct targets of miR-26a. (A) The paired bases of miR-26a with MMP14 and MMP16 and Western blot (B) WT and mutant MMP14 or MMP16 luciferase plasmids were transfected into HEK293 cells with miR-NC or miR-26a. The luciferase activity was measured by dual-luciferase reporter assay system. (*p<0.05). AgomiR-26a or miR-26a inhibitor or its NC was transfected into SCL-1 cells. (C) The mRNA level of MMP14 and MMP16 was analyzed by qRT-PCR (*p<0.01). (D) Western blot was performed to detect MMP14 and MMP16 protein expression (*p<0.05). (E) ELISA assay was used to determine the activity of MMP14 and MMP16 in cell supernatant (*p<0.05). The above measurement data were expressed as mean ± standard deviation. Data among multiple groups were analyzed by one-way ANOVA, followed by a Tukey post hoc test. The experiment was repeated in triplicate.