Table 1.

Benchmarked statistical models to estimate measurement error in fitness scores

Error model type	Based on	Additional input?	Information sharing across variants?	Model parameters
Variance-based	Empirical variance of replicate fitness	No	No	0
Bayesian regularization of variance [51]	Empirical variance of replicate fitness	Yes: Bayesian prior to regression on seq. counts	Yes: prior estimated across all variants	3
Count-based	Sequencing counts follow Poisson distribution	No	No	0
Enrich2 [36]	Sequencing counts follow Poisson distribution	Yes: mixed-effects from empirical variance	No	# variants
DiMSum	Sequencing counts follow Poisson distribution	Yes: modifier terms from empirical variance	Yes: replicate-specific modifier terms estimated across all variants	3 × # replicates