FIGURE 4.

Ultrastructural imaging of pulmonary endothelial injury by transmission electron microscopy. Pulmonary endothelial injury and endothelial glycocalyx injury were morphologically attenuated to a greater extent in recombinant human thrombomodulin (rhTM)‐treated mice than in saline‐injected mice. Conventional imaging (without lanthanum nitrate) of pulmonary endothelium by transmission electron microscopy in (a, d) sham mice and (b, e) saline‐injected and (c, f) rhTM‐treated mice after LPS administration. (d), (e), and (f) are expanded images of the white‐boxed area in (a), (b), and (c) respectively. Glycocalyx imaging (with lanthanum nitrate) by scanning transmission electron microscopy in (g, j) sham mice and (h, k) saline‐injected and (i, l) rhTM‐treated mice after LPS administration. (j), (k), and (l) are expanded images of the white‐boxed area in (g), (h), and (i) respectively