FIGURE 7.

Immunohistochemical analysis of endothelial cell‐specific molecule 1 (ESM 1). (a) Immunohistochemical analysis of ESM 1 in the lungs of sham mice and saline‐injected and recombinant human thrombomodulin (rhTM)‐treated mice after LPS administration. Black arrows indicate ESM 1‐positive endothelial cells. Red arrows indicate ESM 1‐positive inflammatory cells. (b) Graph showing ESM 1‐positive cell numbers (n = 6 each). ^* P < .05, significantly different from sham mice. ⁺ P < .05, significantly different from saline+LPS group. (c) Double immunofluorescence analysis of ESM 1 and CD31. The magnified image is the expanded view of the white‐boxed area in the merged image. HPF, high‐power field