FIGURE 2.

Effects of Δ⁹‐THCA‐A on β‐arrestin recruitment. HEK293‐CB₁‐β‐arrestin Nomad cells were pre‐incubated with Δ⁹‐THCA‐A (1 μM), PD98,059 (5 μM), and SR141716 (1 μM) for 20 min and then treated with CP‐55,940 (2.5 μM) for 24 h. (a) Quantification of β‐arrestin recruitment on living cells at the times indicated are shown by fluorescence intensity changes. (b) Representative images of cytoplasmic fluorescence (4× magnification) and (c) cell confluence, at the endpoint were determined using the IncuCyte system. Control group was taken as 100% confluent. Data are means ± SD; n = 5. ^* P < .05, significantly different as indicated