FIGURE 3.

Effects of Δ⁹‐THCA‐A on ERK1/2 phosphorylation. (a) Western blot analysis showing p‐ERK1/2, total ERK1/2, and actin levels in HEK293‐CB₁‐β‐arrestin Nomad cells and HEK293 cells exposed to increased concentrations of Δ⁹‐THCA‐A and CP‐55,940 for 30 min. (b) Quantification of western blot results from five independent experiments. (c) Western blot analysis in HEK293‐CB₁‐β‐arrestin Nomad cells pre‐stimulated with Δ⁹‐THCA‐A (10 μM) and SR141716 (1 μM) for 5 min and treated with CP‐55,940 (10 μM) for 30 min. (d) Quantification of western blot results from five independent experiments. PMA (100 nM) was used as a positive control of ERK1/2 phosphorylation. Data are means ± SD; n = 5. ^* P < .05, significantly different as indicated