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. 2020 Aug 14;10:13831. doi: 10.1038/s41598-020-70764-x

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© This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of combination treatments on prostate cancer spheroids. (A) Cells were plated on Matrigel-coated 24 well plates. Six drugs (cabozantinib, clofarabine, vinblastine, idarubicin, omacetaxine and sorafenib) were tested on prostate cancer spheroids. These experiments were performed in triplicate and are average of 3 replicates from 3 different wells of a cell culture plate. The pictures are representative images as acquired using an EVOS cell imager. (B) Proliferation of spheroids treated with cabozantinib (CBZ), clofarabine (CFB), vinblastine (VBT), idarubicin (IRB), omacetaxine (OAT) and sorafenib (SRN) measured using AnnexinV/PI staining.