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. 2020 Aug 14;10:13787. doi: 10.1038/s41598-020-69568-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Growth of oyster larvae in 50 environmental treatments arranged by target salinity (left–right) and target temperature (bottom-top). Black lines are lengths of larval shells (n = 3–44), white lines are linear regressions, showing average growth/day. Horizontal dotted lines mark our size classification for late-stage larvae (260 µm). Filled in circles represent proportion of sampled larvae with visible eyespots at all samples > 25%. Vertical dotted lines indicate > 95% mortality. Panel color represents growth rate (µm/day). Numbers denote the day that treatments became competent (first sampling of > 25% eyed larvae).