Table 1.
Primer sequences for qRT-PCR analysis of 19 m6A regulators in lung cancer cell lines (note: F = forward, R = reverse). GAPDH was used as control
| Primer name | Primer sequence (from 5′ to 3′) |
|---|---|
| ALKBH5-F | GCAAGGTGAAGAGCGGCATCC |
| ALKBH5-R | GTCCACCGTGTGCTCGTTGTAC |
| FTO-F | GTTCACAACCTCGGTTTAGTTC |
| FTO-R | CATCATCATTGTCCACATCGTC |
| HNRNPA2B1-F | GCTTAAGCTTTGAAACCACAGA |
| HNRNPA2B1-F | GCTTAAGCTTTGAAACCACAGA |
| IGF2BP1-R | GGGGTGGAATATTTCGGATTTG |
| IGF2BP1-F | GATGAAGGCCATCGAAACTTTC |
| IGF2BP2-F | GATGAACAAGCTTTACATCGGG |
| IGF2BP2-R | GATTTTCCCATGCAATTCCACT |
| IGF2BP3-F | GAGGCGCTTTCAGGTAAAATAG |
| IGF2BP3-R | AATGAGGCGGGATATTTCGTAT |
| KIAA1429-F | GCAACTTCAGGCATTAAGTTCA |
| KIAA1429-R | GTATTGCCTTGTCGAATCTGTC |
| METTL14-F | CAGGCTGGCTCACAGTTGGAC |
| METTL14-R | TTCCACCTCTTCCTCCACCTCTG |
| METTL3-F | CTTCAGCAGTTCCTGAATTAGC |
| METTL3-R | ATGTTAAGGCCAGATCAGAGAG |
| RBM15-F | GGCTGCCTGAGGAGAGTGGAG |
| RBM15-R | CGGCTACTGCTCAATTCTGGACTG |
| RBM15B-F | ATCTTTCAGAGTACGCTCAGAC |
| RBM15B-R | CTAGGATATGCATAGACGTGGG |
| WTAP-F | CTGACAAACGGACCAAGTAATG |
| WTAP-R | AAAGTCATCTTCGGTTGTGTTG |
| YTHDC1-F | AGTGACTCTGGTTCTGAATCTG |
| YTHDC1-R | CTGGTTTGATCTTTTCGGACAG |
| YTHDC2-F | GAGAATTGGGCTGTCGTTAAAG |
| YTHDC2-R | TGAAGCAGGATGAAATCGTACT |
| YTHDF2-F | ACTTCTCAGCATGGGGAAATAA |
| YTHDF2-R | TATTCATGCCAGGAGCCTTATT |
| YTHDF3-F | TCAACCACCACAACCACAGCAG |
| YTHDF3-R | TGAAGCACTGACAGGTACAACACC |
| ZC3H13-F | GATCAGTTAAAGCGTGGAGAAC |
| ZC3H13-R | CTCTCTGTCGTGTTCATATCGA |
| YTHDF1-F | ATGACAATGACTTTGAGCCCTA |
| YTHDF1-R | AGGGAGTAAGGAAATCCAATGG |
| HNRNPC-F | ACAGATCCTCGCTCCATGAACTCC |
| HNRNPC-R | TTCTGCCATCCTCTCCTGCTACAG |
| GAPDH-F | CTGCACCACCAACTGCTT |
| GAPDH-R | TTCTGGGTGGCAGTGATG |