Table 7.
A total of 10 hub molecules in invasive NFPAs identified with PPI analysis of 130 overlapped molecules (invasive DEG; phosphoproteins)
| Protein accession ID | DEG name | DEG level | Protein name | Phosphorylation level | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| logFC | AveExpr | t | P value | Phosphopeptide | Phosphorylated site | Phosphorylated position | Phosphorylated probabilities | PEP | Score | Ratio (T/N) | P value (t test) | |||
| Q8WXD2 | SCG3 | 1.2843 | 10.4326 | 4.3052 | 1.72E-03 | Secretogranin-3 | ELS*AERPLNEQIAEAEEDKIK | S | 37 | 1 | 2.80E-38 | 149 | 0.46 | 1.50E-04 |
| LFPAPS*EKSHEETDSTK | S | 359 | 0.468 | 1.85E-02 | 36 | |||||||||
| P49815 | TSC2 | 2.1795 | 10.7866 | 6.3857 | 9.73E-05 | Tuberin | S*SSSPELQTLQDILGDPGDK | S; S; S | 1385; 1386; 1387 | 0.313; 0.313; 0.313 | 1.56E-11 | 74 | ||
| P02768-1 | ALB | 1.9536 | 6.1289 | 4.5055 | 1.27E-03 | Serum albumin | TCVADES*AENCDK | S | 82 | 1 | 1.24E-47 | 197 | 0.74 | 3.24E-04 |
| P31749 | AKT1 | 1.1801 | 6.8509 | 5.4900 | 3.11E-04 | RAC-alpha serine/threonine-protein kinase | S*GSPSDNSGAEEMEVSLAKPK | S; S | 122; 124 | 0.457; 0.457 | 1.16E-07 | 92 | ||
| A5PL32 | APOL1 | − 1.1936 | 9.8189 | − 3.2480 | 9.26E-03 | Apolipoprotein L1 | VTEPIS*AES*GEQVER | S; S | 352; 355 | 1; 1 | 2.53E-03 | 59 | 0.71 | 5.33E-03 |
| Q13085 | ACACA | 1.1822 | 6.8754 | 3.3664 | 7.62E-03 | Acetyl-CoA carboxylase 1 | FIIGSVSEDNS*EDEISNLVK | S | 29 | 1 | 3.35E-32 | 154 | 2.98 | 6.08E-05 |
| Q14515 | SPARCL1 | − 3.1871 | 13.6134 | − 4.7627 | 8.66E-04 | SPARC-like protein 1 | EES*HEQS*AEQGK | S | 80 | 9.37E-38 | 176 | 0.24 | 1.86E-05 | |
| SSS*QELGLK | S | 92 | 0.999 | 1.61E-28 | 188 | 0.28 | 1.91E-05 | |||||||
| SKEES*HEQS*AEQGK | S; S | 80; 84 | 0.999; 1 | 9.37E-38 | 176 | 0.35 | 5.38E-05 | |||||||
| EHANS*KQEEDNTQSDDILEESDQPTQVSK | S | 231 | 1 | 5.61E-70 | 176 | 0.36 | 5.25E-04 | |||||||
| HIQETEWQS*QEGK | S | 295 | 1 | 2.83E-21 | 152 | 0.57 | 1.15E-03 | |||||||
| DQGNQEQDPNIS*NGEEEEEKEPGEVGTHNDNQER | S | 198 | 0.997 | 1.10E-05 | 53 | 0.89 | 1.92E-01 | |||||||
| EHANS*KQEEDNT*QS*DDILEESDQPTQVSK | S; T; S | 231; 238; 240 | 0.331; 0.331; 0.331 | 1.44E-02 | 38 | |||||||||
| S*KEES*HEQSAEQGK | S; S | 76; 80 | 0.999; 0.995 | 7.77E-05 | 77 | |||||||||
| P14672 | SLC2A4 | − 1.3065 | 5.1362 | − 4.4148 | 1.45E-03 | Solute carrier family 2, facilitated glucose transporter member 4 | T*PSLLEQEVKPSTELEYLGPDEND | T; S | 486; 488 | 0.5; 0.5 | 1.85E-10 | 91 | ||
| P05060 | CHGB | − 1.3288 | 14.5253 | − 3.2997 | 8.51E-03 | Secretogranin-1 | SETHAAGHS*QEK | S | 225 | 1 | 4.87E-30 | 175 | 0.14 | 6.87E-07 |
| GRGS*EEYR | S | 367 | 1 | 1.70E-07 | 146 | 0.12 | 7.46E-07 | |||||||
| AS*EEEPEYGEEIK | S | 335 | 1 | 5.81E-71 | 223 | 0.13 | 2.05E-06 | |||||||
| EKSS*QES*GEETGS*QENHPQESK | S; S; S | 236; 239; 245 | 0.531; 1; 0.965 | 4.20E-73 | 205 | 0.06 | 3.67E-06 | |||||||
| ADEPQWSLYPSDS*QVS*EEVK | S; S | 146; 149 | 0.999; 0.999 | 1.30E-150 | 236 | 0.31 | 5.28E-06 | |||||||
| SSQESGEET*GSQENHPQESK | T | 243 | 0.978 | 1.47E-45 | 161 | 0.02 | 5.36E-06 | |||||||
| GERGEDS*S*EEK | S; S | 182; 183 | 1; 1 | 1.80E-03 | 100 | 0.16 | 7.03E-06 | |||||||
| AS*EEEPEY*GEEIK | S; Y | 335; 341 | 1; 1 | 2.35E-06 | 98 | 0.18 | 8.29E-06 | |||||||
| SSQES*GEETGS*QENHPQESK | S; S | 239; 245 | 0.992; 1 | 4.20E-73 | 205 | 0.06 | 1.01E-05 | |||||||
| S*QREDEEEEEGENYQK | S | 160 | 1 | 1.77E-17 | 140 | 0.25 | 1.17E-05 | |||||||
| SAEFPDFYDS*EEPVSTHQEAENEKDR | S | 626 | 1 | 1.85E-99 | 214 | 0.26 | 1.18E-05 | |||||||
| S*QEES*EEGEEDATS*EVDK | S | 259; 263; 272 | 1; 1; 0.857 | 1.51E-53 | 157 | 0.14 | 1.23E-05 | |||||||
| SSQGGS*LPSEEK | S | 298 | 0.981 | 3.28E-14 | 142 | 0.28 | 1.31E-05 | |||||||
| DPADASEAHES*SSR | S | 98 | 0.966 | 1.32E-30 | 169 | 0.10 | 1.41E-05 | |||||||
| NYPS*LELDK | S | 391 | 1 | 1.30E-04 | 112 | 0.14 | 1.44E-05 | |||||||
| ADEPQWSLYPS*DS*QVSEEVK | S; S | 144; 146 | 0.941; 0.751 | 3.58E-98 | 136 | 0.52 | 1.53E-05 | |||||||
| EKSS*QES*GEET*GSQENHPQESK | S; S; T | 237; 239; 243 | 0.819; 0.935; 0.598 | 1.47E-45 | 161 | 0.05 | 1.53E-05 | |||||||
| SSQGGSLPS*EEK | S | 301 | 0.999 | 5.51E-20 | 128 | 0.25 | 1.88E-05 | |||||||
| S*AEFPDFYDSEEPVSTHQEAENEKDR | S | 617 | 1 | 8.30E-152 | 246 | 0.22 | 2.13E-05 | |||||||
| S*AEFPDFY*DSEEPVSTHQEAENEKDR | Y | 624 | 0.995 | 8.86E-43 | 152 | 0.23 | 2.23E-05 | |||||||
| SQEESEEGEEDAT*SEVDK | T | 271 | 0.973 | 1.38E-88 | 219 | 0.21 | 2.23E-05 | |||||||
| M#AHGY*GEES*EEER | Y; S | 401; 405 | 1; 1 | 2.41E-02 | 51 | 0.23 | 2.34E-05 | |||||||
| ERADEPQWSLYPSDSQVS*EEVK | S | 149 | 1 | 2.83E-147 | 264 | 0.29 | 2.80E-05 | |||||||
| PQS*EES*WDEEDKR | S; S | 377; 380 | 1; 1 | 4.73E-42 | 181 | 0.15 | 3.27E-05 | |||||||
| SAEFPDFYDS*EEPVS*THQEAENEK | S; S | 626; 631 | 0.996; 0.863 | 3.45E-35 | 140 | 0.30 | 3.33E-05 | |||||||
| SS*QGGSLPSEEK | S | 294 | 0.807 | 3.74E-09 | 133 | 0.24 | 3.77E-05 | |||||||
| S*SQGGSLPSEEK | S | 293 | 0.992 | 1.97E-30 | 180 | 0.25 | 4.48E-05 | |||||||
| GHPQEES*EESNVSMASLGEK | S | 311 | 1 | 6.58E-209 | 232 | 0.22 | 5.91E-05 | |||||||
| SAEFPDFYDSEEPVS*T*HQEAENEK | S; T | 631; 632 | 0.591; 0.586 | 3.45E-35 | 124 | 0.31 | 5.94E-05 | |||||||
| EKS*S*QES*GEET*GS*QENHPQESK | S; S; S; T; S | 235; 236; 239; 243; 245 | 0.643; 0.589; 0.589; 0.589; 0.589 | 1.47E-45 | 161 | 0.05 | 7.50E-05 | |||||||
| WQQQGDLQDT*KENR | T | 492 | 1 | 6.83E-05 | 85 | 0.37 | 9.99E-05 | |||||||
| GHPQEES*EESNVS*M#ASLGEK | S | 317 | 0.988 | 1.45E-59 | 184 | 0.29 | 1.70E-04 | |||||||
| ADEPQWSLY*PSDSQVS*EEVK | Y; S | 142; 149 | 0.981; 0.96 | 6.42E-108 | 134 | 0.52 | 1.87E-04 | |||||||
| GHPQEES*EESNVS*MAS*LGEK | S; S; S | 314; 320; 323 | 0.739; 1; 1 | 1.83E-50 | 161 | 0.37 | 4.40E-04 | |||||||
| WAEGGGHS*R | S | 130 | 1 | 4.86E-03 | 80 | 0.21 | 5.96E-04 | |||||||
| GHPQEES*EES*NVS*MASLGEK | S; S; S | 314; 317; 320 | 0.959; 0.959; 0.959 | 8.63E-27 | 145 | 0.31 | 6.63E-04 | |||||||
| CIIEVLSNALS*K | S | 46 | 1 | 3.06E-04 | 94 | 0.40 | 1.63E-02 | |||||||
| DHHS*THYRASEEEPEY*GEEIK | S; T; Y | 329; 330; 341 | 0.355; 0.355; 0.98 | 1.56E-02 | 43 | |||||||||
| DPADASEAHESS*S*RGEAGAPGEEDIQGPTK | S; S | 99; 100 | 0.651; 0.651 | 8.57E-07 | 62 | |||||||||
| LLRDPADAS*EAHESSSR | S | 93 | 0.989 | 5.88E-05 | 88 | |||||||||
| S*ETHAAGHS*QEK | S | 217 | 0.757 | 1.02E-02 | 78 | |||||||||
| P24593 | IGFBP5 | − 2.6828 | 10.6639 | − 4.1430 | 2.20E-03 | Insulin-like growth factor-binding protein 5 | EHEEPT*TSEMAEETYSPK | T | 123 | 0.346 | 2.19E-04 | 56 | ||
DEG, differentially expressed gene; LogFC, log2(fold change). LogFC > = 1, upregulated DEG; LogFC <= − 1, downregulated DEGs; Ratio (T/N), the ratio of phosphorylation level of NFPAs to controls