Skip to main content
. 2020 May 25;39(16):e104136. doi: 10.15252/embj.2019104136

Figure 5. Microglial GPR56 is required for synaptic development in the hippocampus.

Figure 5

  • A
    Representative images show hippocampal vGlut2 and Homer1 immunostaining. White box outlines the region of interest and yellow box shows the regions where confocal images were taken. Scale bar, 500 μm.
  • B
    Confocal images of synaptic immunostaining in CA1 striatum lacunosum‐moleculare (str. lac/mol) at P10. Arrows pointing to the enlarged synapse in the insets. Scale bar, 5 μm.
  • C, D
    Quantification of synapse density in CA1 str. lac/mol in iCKO versus control at P10 (C), and CKO versus control at P21 (D). At P10, N(Ctrl) = 4, N(iCKO) = 3, P = 0.02. At P21, N = 8, P = 0.005.
  • E
    Representative images show hippocampal vGlut1 and Homer1 immunostaining.
  • F
    Confocal images of synaptic immunostaining in CA1 striatum radiatum at P10.
  • G, H
    Quantification of synapse density in CA1 striatum radiatum in iCKO versus control at P10 (G), and CKO versus control at P21 (H). At P10, N = 5, P = 0.83. At P21, N = 5, P = 0.89.
Data information: *P < 0.05, **P < 0.01, Student's t‐test. All data are presented as mean ± SD.