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. 2020 May 25;39(16):e104136. doi: 10.15252/embj.2019104136

Figure EV5. Deleting microglial Gpr56 leads to impaired eye‐specific segregation at P10 and reduced engulfment of RGC inputs by microglia.

Figure EV5

  • A
    The left two columns show contra‐ and ipsilateral RGC inputs labeled by CTB488 and CTB594, respectively. The middle two columns are binary images of contra‐ and ipsilateral LGN. The right images show an overlap between contralateral RGC inputs and ipsilateral inputs. Scale bar, 250 μm.
  • B
    Quantification of the percentage of overlapped contra‐ and ipsilateral RGC inputs on multiple thresholds in CKO and controls at P10. N = 4, ****P < 0.0001, two‐way ANOVA with Bonferroni's post hoc test.
  • C
    Representative images and surface rendered microglia (green) from P5 dLGN of CKO or controls in which RGC inputs were labeled with CTB‐594 (red). Scale bar, 20 μm.
  • D
    Quantification of the percentage of engulfed RGC inputs in controls and CKO microglia. More than 10 microglial cells are analyzed in each individual mouse brain. N = 4, *P = 0.039.
Data information: Mean ± SEM.